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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Kedokteran Syiah Kuala e-Journal Pustaka Kesehatan Research Journal of Life Science Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Mutiara Medika: Jurnal Kedokteran dan Kesehatan UNEJ e-Proceeding MNJ (Malang Neurology Journal) Health Notions Jurnal Pengabdian Pada Masyarakat Warta Pengabdian Multidiciplinary Journal Al-sihah: The Public Health Science Journal Manuju : Malahayati Nursing Journal Majalah Kedokteran Andalas Medical Technology and Public Health Journal Jurnal Inovasi Teknik Kimia Jurnal ABDINUS : Jurnal Pengabdian Nusantara AloHA International Journal of Health Advancement (AIJHA) STRADA Jurnal Ilmiah Kesehatan Jurnal Kesehatan dr. Soebandi
Erma Sulistyaningsih
Department Of Parasitology, Faculty Of Medicine, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Journal : Research Journal of Life Science

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Optimized Expression Condition of CIDRα-PfEMP1 Recombinant Protein Production in Escherichia coli BL21(DE3): A Step to Develop Malaria Vaccine Candidate Winnie Almira Setyoadji; Erma Sulistyaningsih; Irawan Fajar Kusuma
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.3

Abstract

Malaria is still an essential epidemiological disease worldwide, including in Indonesia. Several approaches are performed to control the disease, as well as vaccine development. The Cysteine-rich interdomain region α of Plasmodium falciparum erythrocyte membrane protein 1 (CIDRα-PfEMP1) is a pivotal domain in the malaria pathogenesis make it a malaria vaccine candidate. The development of the malaria vaccine is performed using recombinant technology. Recombinant protein production is an important step. The study aimed to determine the optimized condition for CIDRα-PfEMP1 recombinant protein expression in Escherichia coli BL21(DE3) expression system. Serial IPTG concentrations from 0.05, 0.1, 0.3, and 0.5 mM and two different incubation periods of 4 h and 8 h were optimized. The recombinant protein expression was visualized in SDS-PAGE, measured using the Bradford protein assay, and calculated using software Image J. SDS-PAGE visualization showed a 27 kDa band expressed CIDRα-PfEMP1 recombinant protein. The optimized condition for CIDRα-PfEMP1 recombinant protein expression was at 0.03 mM IPTG concentration and 8 h incubation period.
Co-Authors Agustina Tri Endharti Al Munawir Aldi Cahya Muhammad Alfi Kamalia Aliyatul Farida Anas Bakhtiar Diyansah Ancah Caesarina Novi Marchianti Bagus Hermansyah Bagus Hermansyah Burhan, Niniek Cholis Abrori, Cholis Cicih Komariah Denaneer Rahmadatu Devita Prima Nurmasari Didi Candradikusuma Didi Candradikusuma Dini Agustina Dion Krismashogi Darmawan Dwi Wahyuni Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Endang Sri Purwanti Wulandari Eqiel Navadz Akthar Alami Erfan Efendi Eva Sulawati Arum Fathul Hidayatul Hasanah Febrina Sylva Fridayanti, Febrina Sylva Fifil Sumarni Sumarni Firdha Novitasari Fitri Dika Maharani FX Ady Soesetijo, FX Ady Ghani Silahudin Hadi Prayitno Heni Fatmawati Hidayat Sujuti I Ketut Suada Ika Rahmawati Sutejo Intan Rachmawati Irawan Fajar Kusuma Iza Billa Fahmi Jarot Subandono Jarot Subandono Jauhar Firdaus Josef Sem Berth Tuda Kahfi Karunia Ilahi Laeli Kurniasari Laili, Elisa Fadia Laily Rahmawati Leersia Yusi Ratnawati Loeki Enggar Fitri Luh Putu Ratna Sundari Muhammad Hasan Ngurah Agung Reza Satria Nugraha Putra Nicole Berens-Riha Nicole Berens-Riha, Nicole Niniek Burhan Nur Aqmarina Kusumawardani Pralampita, Pulong Wijang Pungky Setya Arini Rina Fitriana Rina Fitriana Ristya Widi Endah Yani Rosita Dewi Salim Salim Samsul Arifin Samsul Arifin Sheilla Rachmania Silvi Ahmada Chasya Sinta Ariyani Sri Hernawati Sugiyanta Sugiyanta Sukarti Moeljopawiro Sukarti Moeljopawiro Thomas Loescher Thomas Loescher, Thomas Tristira Urvina Tuda, Josef Sem Berth Umar Zein Warda Ayu Nadira Wayan T. Artama Wayan T. Artama Widhi Dyah Sawitri Wiji Utami Winnie Almira Setyoadji Wiwien Sugih Utami Yunita Armiyanti